Bus Amsterdam to Leiden, Darwinweg/Naturalis from €14 | Tickets & Timetables

Amsterdam to Leiden, Darwinweg/Naturalis buses

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Trip Overview

The average bus between Amsterdam and Leiden, Darwinweg/Naturalis takes 1h 31m and the fastest bus takes 1h 17m. There is an hourly bus service from Amsterdam to Leiden, Darwinweg/Naturalis. The journey time may be longer on weekends and holidays; use the search form on this page to search for a specific travel date.

1h 31m
Duration: 49 km
Distance: €14
Average price: Hourly
Frequency

Line 397 bus, line 365 bus

best

Take the bus from Amsterdam, Busstation Elandsgracht to Schiphol, Airportbus 397 / ...
Take the bus from Schiphol, Airport/Plaza to Leiden, Leiden CS Westzijdebus 365

1h 59m

€13–16

Amsterdam to Leiden, Darwinweg/Naturalis bus times

Buses run hourly between Amsterdam and Leiden, Darwinweg/Naturalis. The earliest departure is at 5:15 AM in the morning, and the last departure from Amsterdam is at 11:07 PM which arrives into Leiden, Darwinweg/Naturalis at 12:30 AM. All services require a transfer at Schiphol, Airport/Plaza and take an average of 1h 31m. The schedules shown below are for the next available departures.

Departing Saturday, August 22, 2026

5:15 AM → 6:37 AM
1h 22m • 1 change
6:50 AM → 8:07 AM
1h 17m • 1 change
8:10 AM → 9:34 AM
1h 24m • 1 change
9:10 AM → 10:37 AM
1h 27m • 1 change
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Departing Sunday, August 23, 2026

7:14 AM → 8:33 AM
1h 19m • 1 change
8:14 AM → 9:33 AM
1h 19m • 1 change
9:14 AM → 10:33 AM
1h 19m • 1 change
10:04 AM → 11:33 AM
1h 29m • 1 change
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Departing Monday, August 24, 2026

5:15 AM → 6:35 AM
1h 20m • 1 change
5:45 AM → 7:10 AM
1h 25m • 1 change
6:19 AM → 7:47 AM
1h 28m • 1 change
6:52 AM → 8:23 AM
1h 31m • 1 change
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Frequently asked questions

Want to know about travelling from Amsterdam to Leiden, Darwinweg/Naturalis? We have put together a list of the most frequently asked questions from our users such as: What is the cheapest mode of transport? What is the quickest option? How much do tickets usually cost? and many more.