The average bus between Daytona Beach and Gainesville takes 5h 33m and the fastest bus takes 4h 40m. The bus service runs several times per day from Daytona Beach to Gainesville. The journey time may be longer on weekends and holidays; use the search form on this page to search for a specific travel date.
Buses run twice daily between Daytona Beach and Gainesville. The earliest departure is at 1:55 PM in the afternoon, and the last departure from Daytona Beach is at 11:25 PM which arrives into Gainesville at 5:05 AM. All services require a transfer, and take on average 5h 33m. The schedules shown below are for the next available departures.
Want to know about travelling from Daytona Beach to Gainesville? We have put together a list of the most frequently asked questions from our users such as: What is the cheapest mode of transport? What is the quickest option? How much do tickets usually cost? and many more.
No, there is no direct bus from Daytona Beach to Gainesville. However, there are services departing from Daytona Beach station and arriving at Gainesville station via Orlando Bus Station station. The journey, including transfers, takes approximately 5h 33m.
Daytona Beach to Gainesville bus services, operated by Jefferson Lines, depart from Daytona Beach Bus Station.
Daytona Beach to Gainesville bus services, operated by Jefferson Lines, arrive at Gainesville station.
Book your Daytona Beach to Gainesville bus tickets online with Jefferson Lines.
The distance between Daytona Beach and Gainesville is 135.3 km. The road distance is 265 km.
You can take a bus from Daytona Beach Bus Station to Gainesville via Orlando Bus Station in around 4h 20m.
