The average bus between Downtown Orlando and Gainesville takes 3h 21m and the fastest bus takes 2h 40m. The bus service runs several times per day from Downtown Orlando to Gainesville. The journey time may be longer on weekends and holidays; use the search form on this page to search for a specific travel date.
Buses run 4 times a day between Downtown Orlando and Gainesville. The earliest departure is at 8:15 AM in the morning, and the last departure from Downtown Orlando is at 9:50 PM which arrives into Gainesville at 3:05 AM. All services require a transfer at N John Young Pkwy & Business Center Blvd and take an average of 3h 21m. The schedules shown below are for the next available departures.

3h 10m • 1 change
2h 50m • 1 change
2h 45m • 1 change
3h 10m • 1 change
2h 40m • 1 change
2h 50m • 1 change
2h 45m • 1 change
3h 10m • 1 change
3h 10m • 1 change
2h 50m • 1 change
2h 45m • 1 change
3h 10m • 1 changeWant to know about travelling from Downtown Orlando to Gainesville? We have put together a list of the most frequently asked questions from our users such as: What is the cheapest mode of transport? What is the quickest option? How much do tickets usually cost? and many more.
No, there is no direct bus from Downtown Orlando to Gainesville. However, there are services departing from Orlando Bus Station and arriving at Gainesville station via Gainesville station. The journey, including transfers, takes approximately 3h 21m.
Downtown Orlando to Gainesville bus services, operated by Flixbus USA, depart from Orlando Bus Station.
Downtown Orlando to Gainesville bus services, operated by Flixbus USA, arrive at Gainesville station.
The distance between Downtown Orlando and Gainesville is 154.8 km. The road distance is 180 km.
Flixbus USA operates a bus from Orlando Bus Station to Gainesville 4 times a day. Tickets cost $17–26 and the journey takes 2h 10m.