The average bus between Florence and Rome Termini takes 4h 5m and the fastest bus takes 3h 33m. There is a bus service every few hours from Florence to Rome Termini. The journey time may be longer on weekends and holidays; use the search form on this page to search for a specific travel date.
Buses run hourly between Florence and Rome Termini. The earliest departure is at 2:20 AM at night, and the last departure from Florence is at 11:10 PM which arrives into Rome Termini at 5:28 AM. All services require a transfer, and take on average 4h 5m. The schedules shown below are for the next available departures.

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3h 51m • 1 changeWant to know about travelling from Florence to Rome Termini? We have put together a list of the most frequently asked questions from our users such as: What is the cheapest mode of transport? What is the quickest option? How much do tickets usually cost? and many more.
No, there is no direct bus from Florence to Rome Termini. However, there are services departing from Florence Villa Constanza Bus Station and arriving at Roma, Autostazione Tiburtina station via Roma, Autostazione Tiburtina station. The journey, including transfers, takes approximately 4h 5m.
Florence to Rome Termini bus services, operated by FlixBus, depart from Florence Villa Constanza Bus Station.
Florence to Rome Termini bus services, operated by FlixBus, arrive at Roma, Autostazione Tiburtina station.
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The distance between Florence and Rome Termini is 232.4 km. The road distance is 274 km.
FlixBus operates a bus from Florence Villa Constanza Bus Station to Roma, Autostazione Tiburtina every 30 minutes. Tickets cost €24–40 and the journey takes 3h 15m. Three other operators also service this route.