The average bus between Florence and Vatican Museums takes 4h 35m and the fastest bus takes 4h. There is a bus service every few hours from Florence to Vatican Museums. The journey time may be longer on weekends and holidays; use the search form on this page to search for a specific travel date.
Buses run hourly between Florence and Vatican Museums. The earliest departure is at 2:20 AM at night, and the last departure from Florence is at 11:10 PM which arrives into Vatican Museums at 5:54 AM. All services require a transfer at Roma, Autostazione Tiburtina and take an average of 4h 35m. The schedules shown below are for the next available departures.

5h 1m • 1 change

4h 7m • 1 change
Arrives next day

4h 15m • 1 change
Arrives next day

6h 44m • 1 change

4h 43m • 1 change

4h 16m • 1 change

5h 4m • 1 change

4h 18m • 1 change

4h 38m • 1 change

4h 10m • 1 change

5h • 1 change

4h 23m • 1 change

4h 38m • 1 change

4h 10m • 1 change

5h • 1 change

4h 23m • 1 change
Want to know about travelling from Florence to Vatican Museums? We have put together a list of the most frequently asked questions from our users such as: What is the cheapest mode of transport? What is the quickest option? How much do tickets usually cost? and many more.
No, there is no direct bus from Florence to Vatican Museums. However, there are services departing from Florence station and arriving at Vatican Museums station via Roma, Autostazione Tiburtina. The journey, including transfers, takes approximately 4h 35m.
Florence to Vatican Museums bus services, operated by FlixBus, depart from Florence Villa Constanza Bus Station.
Florence to Vatican Museums bus services, operated by FlixBus, arrive at Roma, Autostazione Tiburtina station.
The distance between Florence and Vatican Museums is 230.5 km. The road distance is 289 km.
FlixBus operates a bus from Florence Villa Constanza Bus Station to Roma, Autostazione Tiburtina hourly. Tickets cost €24–40 and the journey takes 3h 15m. Three other operators also service this route.