Gainesville to Universal Studios Florida Buses

Gainesville to Universal Studios Florida buses

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Trip Overview

The average bus between Gainesville and Universal Studios Florida takes 4h 41m and the fastest bus takes 2h 57m. The bus service runs several times per day from Gainesville to Universal Studios Florida. The journey time may be longer on weekends and holidays; use the search form on this page to search for a specific travel date.

4h 41m
Duration: 122 mi
Distance: $23
Average price: 5 times a day
Frequency

Bus via N Garland Ave & W Amelia St (Flixbus)

best

Take the bus from Gainesville to Orlando Bus Stationbus FlixBus 2415 / ...

3h 30m

$20–28

Gainesville to Universal Studios Florida bus times

Buses run 5 times a day between Gainesville and Universal Studios Florida. The earliest departure is at 3:45 AM at night, and the last departure from Gainesville is at 9:15 PM which arrives into Universal Studios Florida at 6:03 AM. All services require a transfer at Orlando Bus Station and take an average of 4h 41m. The schedules shown below are for the next available departures.

Departing Monday, November 2, 2026

9:15 PM → 6:03 AM
Arrives next day
8h 48m • 2 changes

Departing Tuesday, November 3, 2026

3:45 AM → 7:03 AM
3h 18m • 2 changes
5:20 AM → 9:04 AM
3h 44m • 2 changes
12:05 PM → 3:35 PM
3h 30m • 2 changes
9:15 PM → 6:03 AM
Arrives next day
8h 48m • 2 changes

Departing Wednesday, November 4, 2026

3:45 AM → 7:03 AM
3h 18m • 2 changes
5:20 AM → 9:04 AM
3h 44m • 2 changes
7:00 PM → 6:03 AM
Arrives next day
11h 3m • 2 changes

Departing Thursday, November 5, 2026

3:45 AM → 7:03 AM
3h 18m • 2 changes
4:15 AM → 7:34 AM
3h 19m • 2 changes
5:20 AM → 9:04 AM
3h 44m • 2 changes
12:05 PM → 3:35 PM
3h 30m • 2 changes
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Departing Friday, November 6, 2026

3:45 AM → 7:03 AM
3h 18m • 2 changes
4:15 AM → 7:34 AM
3h 19m • 2 changes
5:20 AM → 9:04 AM
3h 44m • 2 changes
12:05 PM → 3:35 PM
3h 30m • 2 changes
Show more

Departing Saturday, November 7, 2026

3:45 AM → 7:02 AM
3h 17m • 2 changes
4:15 AM → 7:32 AM
3h 17m • 2 changes
5:20 AM → 9:02 AM
3h 42m • 2 changes
12:05 PM → 3:02 PM
2h 57m • 2 changes
Show more

Departing Sunday, November 8, 2026

3:45 AM → 7:02 AM
3h 17m • 2 changes
4:15 AM → 8:02 AM
3h 47m • 2 changes
5:20 AM → 9:02 AM
3h 42m • 2 changes
12:05 PM → 3:02 PM
2h 57m • 2 changes

Frequently asked questions

Want to know about travelling from Gainesville to Universal Studios Florida? We have put together a list of the most frequently asked questions from our users such as: What is the cheapest mode of transport? What is the quickest option? How much do tickets usually cost? and many more.