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La Crosse to Gainesville buses

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Trip Overview

The average bus between La Crosse and Gainesville takes 35h 41m and the fastest bus takes 32h 45m. The bus runs, on average, 6 times per week from La Crosse to Gainesville. The journey time may be longer on weekends and holidays; use the search form on this page to search for a specific travel date.

35h 41m
Duration
1,341 mi
Distance
$309
Average price
6 times a week
Frequency

La Crosse to Gainesville bus times

Buses run 6 times a week between La Crosse and Gainesville. The service departs La Crosse at 2:30 PM in the afternoon, which arrives into Gainesville at 03:40:00. All services require a transfer at Milwaukee and take an average of 35h 41m. The schedules shown below are for the next available departures.

Departing Sunday, November 8, 2026

  •  → 

    Arrives +2 days

    Jefferson LinesGreyhound USAGreyhound USAFlixbus USA37h 10m • 3 changes

Departing Monday, November 9, 2026

  •  → 

    Arrives +2 days

    Jefferson LinesGreyhound USAGreyhound USAFlixbus USA37h 10m • 3 changes

Departing Tuesday, November 10, 2026

  •  → 

    Arrives +2 days

    Jefferson LinesGreyhound USAGreyhound USAFlixbus USA37h 10m • 3 changes

Departing Wednesday, November 11, 2026

  •  → 

    Arrives +2 days

    Jefferson LinesGreyhound USAGreyhound USAFlixbus USA37h 10m • 3 changes

Departing Thursday, November 12, 2026

  •  → 

    Arrives next day

    Jefferson LinesGreyhound USAGreyhound USAFlixbus USA32h 45m • 3 changes

Departing Friday, November 13, 2026

  •  → 

    Arrives next day

    Jefferson LinesGreyhound USAGreyhound USAFlixbus USA32h 45m • 3 changes

Frequently asked questions

Want to know about travelling from La Crosse to Gainesville? We have put together a list of the most frequently asked questions from our users such as: What is the cheapest mode of transport? What is the quickest option? How much do tickets usually cost? and many more.

Map of how to get from La Crosse to Gainesville.ExpandLaunch map view
  • Distance: 1,340.6 mi
  • Duration: 35h 41m

What companies run services between La Crosse, WI, USA and Gainesville, FL, USA?

You can take a bus from La Crosse to Gainesville via Milwaukee, Milwaukee Intermodal Station, Chicago Bus Station, and Atlanta Bus Station in around 29h 30m.

Operators