The average bus between Rome Vatican and Florence takes 4h 21m and the fastest bus takes 4h 1m. There is a bus service every few hours from Rome Vatican to Florence. The journey time may be longer on weekends and holidays; use the search form on this page to search for a specific travel date.
Buses run hourly between Rome Vatican and Florence. The earliest departure is at 6:09 AM in the morning, and the last departure from Rome Vatican is at 9:49 PM which arrives into Florence at 2:10 AM. All services require a transfer, and take on average 4h 21m. The schedules shown below are for the next available departures.

4h 34m • 1 change

5h • 1 change

4h 30m • 1 change

4h 45m • 1 change

4h 5m • 1 change

4h 28m • 1 change

4h 15m • 1 change

4h 17m • 1 change

4h 3m • 1 change

4h 10m • 1 change

4h 2m • 1 change

4h 19m • 1 change
Want to know about travelling from Rome Vatican to Florence? We have put together a list of the most frequently asked questions from our users such as: What is the cheapest mode of transport? What is the quickest option? How much do tickets usually cost? and many more.
No, there is no direct bus from Rome Vatican to Florence. However, there are services departing from Rome Vatican station and arriving at Florence station via Florence Villa Constanza Bus Station station. The journey, including transfers, takes approximately 4h 21m.
Rome Vatican to Florence bus services, operated by ATAC S.p.A. Azienda per la mobilità, depart from Roma, Autostazione Tiburtina station.
Rome Vatican to Florence bus services, operated by ATAC S.p.A. Azienda per la mobilità, arrive at Florence Villa Constanza Bus Station.
The distance between Rome Vatican and Florence is 230.8 km. The road distance is 286 km.
FlixBus operates a bus from Roma, Autostazione Tiburtina to Florence Villa Constanza Bus Station hourly. Tickets cost €24–40 and the journey takes 3h 15m. Three other operators also service this route.