Universal Studios Florida to Gainesville Buses

Universal Studios Florida to Gainesville buses

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Trip Overview

The average bus between Universal Studios Florida and Gainesville takes 3h 58m and the fastest bus takes 3h 8m. The bus service runs several times per day from Universal Studios Florida to Gainesville. The journey time may be longer on weekends and holidays; use the search form on this page to search for a specific travel date.

3h 58m
Duration: 121 mi
Distance: $21
Average price: 4 times a day
Frequency

Bus via N Garland Ave & W Amelia St (Flixbus)

best

Take the bus from Orlando Bus Station to Gainesvillebus FlixBus 2415 / ...

$18–27

Universal Studios Florida to Gainesville bus times

Buses run 4 times a day between Universal Studios Florida and Gainesville. The earliest departure is at 7:35 AM in the morning, and the last departure from Universal Studios Florida is at 9:07 PM which arrives into Gainesville at 3:05 AM. All services require a transfer at Universal Blvd & Hollywood Way and take an average of 3h 58m. The schedules shown below are for the next available departures.

Departing Friday, October 16, 2026

9:02 PM → 3:05 AM
Arrives next day
6h 3m • 2 changes

Departing Saturday, October 17, 2026

8:05 AM → 11:25 AM
3h 20m • 2 changes
12:05 PM → 3:35 PM
3h 30m • 2 changes
1:35 PM → 5:10 PM
3h 35m • 2 changes
8:07 PM → 11:45 PM
3h 38m • 2 changes
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Departing Sunday, October 18, 2026

7:35 AM → 11:25 AM
3h 50m • 2 changes
11:35 AM → 3:35 PM
4h • 2 changes
1:35 PM → 5:10 PM
3h 35m • 2 changes
8:37 PM → 11:45 PM
3h 8m • 2 changes

Departing Monday, October 19, 2026

8:05 AM → 11:25 AM
3h 20m • 2 changes
12:05 PM → 3:35 PM
3h 30m • 2 changes
1:36 PM → 5:10 PM
3h 34m • 2 changes
8:02 PM → 11:45 PM
3h 43m • 2 changes
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Departing Tuesday, October 20, 2026

8:05 AM → 11:25 AM
3h 20m • 2 changes
1:36 PM → 5:10 PM
3h 34m • 2 changes
9:02 PM → 3:05 AM
Arrives next day
6h 3m • 2 changes

Departing Wednesday, October 21, 2026

8:05 AM → 11:25 AM
3h 20m • 2 changes
12:05 PM → 3:35 PM
3h 30m • 2 changes
1:36 PM → 5:10 PM
3h 34m • 2 changes
9:02 PM → 3:05 AM
Arrives next day
6h 3m • 2 changes

Departing Thursday, October 22, 2026

8:05 AM → 11:25 AM
3h 20m • 2 changes
12:05 PM → 3:35 PM
3h 30m • 2 changes

Frequently asked questions

Want to know about travelling from Universal Studios Florida to Gainesville? We have put together a list of the most frequently asked questions from our users such as: What is the cheapest mode of transport? What is the quickest option? How much do tickets usually cost? and many more.

Universal Studios Florida to Gainesville buses

Trip Overview

Bus via N Garland Ave & W Amelia St (Flixbus)