The average train between Brno and Karlštejn takes 3h 49m and the fastest train takes 3h 25m. There is a train service every few hours from Brno to Karlštejn. The journey time may be longer on weekends and holidays; use the search form on this page to search for a specific travel date.
Trains run hourly between Brno and Karlštejn. The earliest departure is at 3:10 AM at night, and the last departure from Brno is at 9:46 PM which arrives into Karlštejn at 1:11 AM. All services require a transfer, and take on average 3h 49m. The schedules shown below are for the next available departures.

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3h 48m • 1 change
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3h 48m • 1 changeWant to know about travelling from Brno to Karlštejn? We have put together a list of the most frequently asked questions from our users such as: What is the cheapest mode of transport? What is the quickest option? How much do tickets usually cost? and many more.
No, there is no direct train from Brno to Karlštejn. However, there are services departing from Brno station and arriving at Karlštejn station via Praha Hlavni Nadrazi. The journey, including transfers, takes approximately 3h 49m.
Brno to Karlštejn train services, operated by RegioJet, depart from Brno Hlavni Nadrazi station.
Brno to Karlštejn train services, operated by RegioJet, arrive at Karlstejn station.
Book your Brno to Karlštejn train tickets online with RegioJet, České dráhy (ČD) and 3 other operators.
The distance between Brno and Karlštejn is 193.2 km. The road distance is 286 km.
You can take a train from Brno Hlavni Nadrazi to Karlstejn via Praha Hlavni Nadrazi in around 3h 28m.